Suggested by C_F

When inputting a high score the player selects a gender, one of which is 'Other'

Suggested by swrspin

Boss Flea is gender nonconforming, and is quoted as saying "Male, female, what's the difference?"

According to Takashi Iizuka, "NiGHTS is neutral, and therefore has no gender. The impressions of the character with regards to gender are totally up to the player." Source

Titania and the fae folk are referred to by they/them pronouns.

The deity Vivec is intersex and genderfluid.

Suggested by MagneticBurn

Serenade is genderless, described by writer Masakazu Eguchi as being soft, tolerant, with a compassionate heart, ultimate strength by combining masculine and feminine parts; the ultimate existence transcending gender. In the English port Serenade is addressed as 'he' but most characters who refer to them as such have never seen Serenade. Additionally in the Viz English localisation of the NT Warrior manga, Serenade is addressed as 'she' in the first chapter, and as 'he' from then on.

Suggested by smaench

Your rival Jamie is androgynous, uses they/them pronouns, and can be married by male and female players

Suggested by fweged

The Magypsies are described by Alec as being neither men nor women, though the fan-translated version refers to them by male and female pronouns. The Magypsies themselves fall squarely into the role of stereotypical okama, presenting as simultaneously masculine and feminine.

Suggested by bea

Minogame is non-binary, going by they/them pronouns. The manual refers to them as male for 'convenience purposes'.

Suggested by Erato_Heti

Kind of a weird case. In 2016 the option for The Player to be gender-neutral and use they/them pronouns was added. Gender in OFF is generally unstable for some characters, and the Elsen are not specifically gendered at all.

Captain Viridian is referred to by creator Terry Cavanaugh as both she/her and he/him.

Suggested by fweged

Protagonist Lamb is described as 'a guy that wears girl clothes,' and characters express confusion towards Lamb's gender.

Protagonist Dominique Pamplemousse is genderqueer.

Suggested by spin

As of December 2019, the Body Swap mode in Shovel of Hope allows the selection of gender-neutral pronouns alongside body shape selection.

Protagonist can go by they/them pronouns. Tomcat is genderfluid, Sylvan and Turing are non-binary; all go by they/them pronoun except Turing who uses he/him pronouns.

Post-launch update added the ability to use they/them or custom pronouns for Sims. Sims can have customised clothing preferences, body types, reproductive roles, toilet use, walking styles, and speech.

Suggested by fweged

Edge case: Recruitable party member Queen Roger is a crossdressing male who operates a brothel with other crossdressing males. His bio states he was outcasted as a child for his feminine behaviour.

Non-binary pronoun option.

Frisk is referred to by the singular they in game and by Toby Fox, and it intended to be a genderless blank slate for the player to project themselves onto.

Chara is genderless, and is referred to as 'it'.

Napstablook is non-binary.

Mettaton EX/NEO's design pulls from androgynous glam rockstars.

Gender for the player character is seemingly fluid, and the player is referred to by they/them pronouns. You can select any appearance and any voice, with these and your name able to be changed at any time during the game.

Player can choose they/them pronouns.

Suggested by fweged

Niko is stated by Eliza Velasquez to have an ambiguous gender, and by Nightmargin to have an unknown gender

The player character is agender.

Option for player character to use gender neutral pronouns, which can be changed at any time in the settings.

The player can choose the gender marker 'NB'.

Player character can identify as 'other.' This results in Mr. Mackey calling the player's parents to say "Your child just let me know that he, er, they identify as being gender neutral. [...] It's fine, it's just everyone was under the impression that they were a boy." After hanging up the phone, Mr. Mackey says, "This is a shocker, New Kid, but there's nothing wrong with not identifying as a binary gender. You can be whatever sex you want, OK, even if it's none. That's fine!" After leaving Mr. Mackey's office, a group of "rednecks" in a pickup truck drive up and say, "Well, well, well, if it ain't one of them gender neutrals? We don't take kindly to your types around here. Let's welcome this thing to our town," and a battle commences.

Suggested by fweged

Charles Eyler's bio states he is genderfluid, though he is only referred to by he/him pronouns ingame.

They/them pronoun option for player character.

Player character Oz is canonically non-binary and uses he/him pronouns.

Choice of pronouns for the player character and their lover.

Suggested by ZapRowsdower

The player character 'The Bard' is nonbinary and uses any pronouns according to the official tumblr.

As of June 2020, there is an 'Identity' feature available which gives your character the possibility of their already-set identity being non-cis/non-het, with two of those identities being non-binary and genderqueer. You can also explicitly choose a gender identity for your character. If you choose genderqueer or non-binary you are forced to use zie/zir pronouns.

A lot about this game squicks me out!

The player can choose to go by they/them pronouns and chuds on Steam didn't like that I guess.

Kris is genderless.

Chaos is nonbinary.

Bloodhound is non-binary and uses they/them pronouns.

The Hearthians are genderless and use they/them pronouns.

The player can choose to be non-binary. Setsu and Raqio are non-binary and go by they/them pronouns in the English localisation. The original release had an event in-game that treated a non-binary character's biological sex as a plot reveal, but this has since been relocalised.

FL4K, though a robot, uses they/them pronouns and wears a non-binary flag pin.

Player characters can use they/them pronouns.

The protagonist and their rival's gender can be chosen, including a non-binary option.

Player character can equip any type of clothing and hair and is not explicitly gendered.

Suggested by AG147

Secondary protagonist Cam Stone is non-binary and is portrayed by non-binary actor Asia Kate Dillon.

Suggested by ResidentMelville

Petronella, Rook, and Ima are non-binary

Player character Oz is canonically non-binary and uses he/him pronouns. Other non-binary characters include Milo, Hex (one of the love interest options), Sawyer (DLC player character), and Moss (minor NPC).

Suggested by smaench

You can be non-binary and Ronald Reagan will respect your pronouns. Fuck this lol

Suggested by fweged

Centres around two non-binary characters.

Player character is never gendered, and characters Fel and Reed are non-binary.

All characters are referred to by they/them pronouns.

Suggested by tdstr

Player character is non-binary

Testament is nonbinary and uses they/them pronouns.

Suggested by Erato_Heti

Player can choose and customise their pronouns, and character Olive is non-binary, using they/them pronouns.

Niko is non-binary and uses they/them pronouns.

Suggested by ResidentMelville

Sawyer, Rowan, and Holmes are non-binary

Playable character Underling is referred to by they/them pronouns.

The player character can use they/them pronouns, and can change their name and pronouns at any time. Some townsfolk are non-binary or gender-nonconforming.

Character creator includes option for they/them pronouns.

Player character options include they/them pronouns and an androgynous body type. Character Quinn is non-binary.

Suggested by spin

Player is of unspecified gender and is referred to by they/them pronouns.

Suggested by gomit
The player character, 'The Unnamed,' is non-binary.

Suggested by zenoslime

The Tarnished is never gendered, with the option of assigning any voice to any body type. Thus, the player can be of any identity they wish without the game imposing an identity upon them.

Marika and Radagon, although appearing as female and male respectively and being of two wills, share the same physical body. This doesn't necessarily make them intersex but they are reminiscent of the alchemical Rebis, a being of male and female qualities, described as 'the divine hermaphrodite'.

Suggested by romdocitizen

Non-binary supporting character and the player character is implicitly non-binary themselves.

The player character can be non-binary with they/them pronouns, and character Bobbee goes by they/them pronouns.

Suggested by Cold_Comfort and corroborated by Luna, the Endless Witch

Juniper is is not specified as any gender identity in-game unlike other main characters, but they use they/them pronouns and are marked internally with the gender undefined value of 2. Also voiced by an NB in the English localisation.

Suggested by Clearin

The Boss is never assigned a gender in-game and is referred to exclusively as they, them, and you. This allows for non-binary character creation in a way that Cyberpunk 2077 did not (there you would still be labelled male or female).

Suggested by Rowan1312

The player character can choose their pronouns, physical appearance, and reproductive anatomy. Pronoun options include they/them as well as customisable pronouns. Reproductive anatomy comes into play for a puberty event in the game, and the player can opt out of the event being gendered. Selecting a particular reproductive anatomy or appearance does not lead to this event assuming a gender at all. eg. Wet dreams and menstruation are discussed depending on the reproductive anatomy chosen, but they are presented in a gender-neutral and non-judgemental way. There is also a non-binary NPC.

Suggested by Archagent

Character Sam is non-binary and goes by they/them pronouns.

Like Animal Crossing: New Horizons, the player character has a 'style' rather than gender. Clothing and haircuts are unisex.

Regardless of the player's chosen appearance, they are given the option to select pronouns diegetically after the prologue.

Suggested by Archagent

The player character can be non-binary.

Main character Fang goes by they/them pronouns and is voiced by a non-binary VA.